Rabbit anti-Smoothened Antibody

• Background:

  Rabbit polyclonal antibody to Smoothened

• Synonyms:

  SMOH; Smoothened homolog; SMO; Protein Gx

• Host:

  Rabbit

• Reactivity:

  H, M

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Smoothened. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Smoothened expression in HeLa (A) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  6608

• Gene ID (Mouse):

  319757

• SwissProt (Human):

  Q99835

• SwissProt (Mouse):

  P56726

• CAS Number:

  9007-83-4