Rabbit anti-SMAD1 Antibody

• Background:

  Rabbit polyclonal antibody to SMAD1

• Synonyms:

  SMAD1; BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD5; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD9; MADH6; MADH9; Mothers against decapentaplegic homolog 9; MAD homolog 9; Mothers against DPP homolog 9; Madh6; SMAD family member 9; SMAD 9; Smad9

• Host:

  Rabbit

• Reactivity:

  H, M, R, B, C, Z

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SMAD1. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of SMAD1 expression in Jurkat (A), HeLa (B), Ramos (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of SMAD1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  4086; 4090; 4093

• Gene ID (Mouse):

  17125; 17129; 55994

• Gene ID (Rat):

  59328; 85435

• SwissProt (Human):

  Q15797; Q99717; O15198

• SwissProt (Mouse):

  P70340; P97454; Q9JIW5

• SwissProt (Rat):

  P97588; Q9R1V3; O54835

• CAS Number:

  9007-83-4