Rabbit anti-SAP49 Antibody

• Background:

  Rabbit polyclonal antibody to SAP49

• Synonyms:

  SAP49; Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SF3b50; Spliceosome-associated protein 49; SAP 49

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SAP49. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Rabbit

• WB Description:

  Western blot analysis of SAP49 expression in HEK293T (A), Jurkat (B) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of SAP49 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  10262

• Gene ID (Mouse):

  107701

• Gene ID (Rat):

  295270

• SwissProt (Human):

  Q15427

• SwissProt (Mouse):

  Q8QZY9

• SwissProt (Rat):

  Q6AYL5

• CAS Number:

  9007-83-4