Rabbit anti-RUNX2 Antibody

• Background:

  Rabbit polyclonal antibody to RUNX2

• Synonyms:

  AML3; CBFA1; OSF2; PEBP2A; Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit

• CAS Number:

  9007-83-4

• Host:

  Rabbit

• Reactivity:

  H, M, R, C

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RUNX2. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Shipping Conditions:

  FALSE

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of RUNX2 expression in Saos2 (A), HepG2 (B), Jurkat (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of RUNX2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  860

• Gene ID (Mouse):

  12393

• SwissProt (Human):

  Q13950

• SwissProt (Mouse):

  Q08775

• SwissProt (Rat):

  Q9Z2J9