Rabbit anti-RAB27 Antibody

• Background:

  Rabbit polyclonal antibody to RAB27

• Synonyms:

  RAB27; Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human RAB27

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of RAB27 expression in Hela (A), U937 (B), Jurkat (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of RAB27 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  5873

• Gene ID (Mouse):

  11891

• Gene ID (Rat):

  50645

• SwissProt (Human):

  P51159

• SwissProt (Mouse):

  Q9ERI2

• SwissProt (Rat):

  P23640

• CAS Number:

  9007-83-4