Rabbit anti-INI1 Antibody

• Background:

  Rabbit polyclonal antibody to INI1

• Synonyms:

  BAF47; INI1; SNF5L1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; BRG1-associated factor 47; BAF47; Integrase interactor 1 protein; SNF5 homolog; hSNF5

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human INI1

• Clonality:

  Polyclonal

• Applications:

  WB, IHC, IF/IC, IP, ChIP

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/50 - 1/200), ChIP (1/20 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Rabbit

• WB Description:

  Western blot analysis of INI1 expression in HepG2 (A), MCF7 (B), mouse brain (C), mouse lung (D) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of INI1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunofluorescent analysis of INI1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  6598

• Gene ID (Mouse):

  20587

• SwissProt (Human):

  Q12824

• SwissProt (Mouse):

  Q9Z0H3

• CAS Number:

  9007-83-4