Rabbit anti-PI3K p110 alpha Antibody

• Background:

  Rabbit polyclonal antibody to PI3K p110 alpha

• Synonyms:

  Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p110 alpha. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of PI3K p110 alpha expression in HeLa (A), mouse liver (B) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of PI3K p110 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  5290

• Gene ID (Mouse):

  18706

• SwissProt (Human):

  P42336

• SwissProt (Mouse):

  P42337

• CAS Number:

  9007-83-4