Rabbit anti-PCNA Antibody

• Background:

  Rabbit polyclonal antibody to PCNA

• Synonyms:

  Proliferating cell nuclear antigen; PCNA; Cyclin

• Host:

  Rabbit

• Reactivity:

  H, M, R, B, C, D, Mk, P, Rb

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Rabbit

• WB Description:

  Western blot analysis of PCNA expression in HeLa (A), SP2/0 (B), H9C2 (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  5111

• Gene ID (Mouse):

  18538

• Gene ID (Rat):

  25737

• SwissProt (Human):

  P12004

• SwissProt (Mouse):

  P17918

• SwissProt (Rat):

  P04961

• CAS Number:

  9007-83-4