Rabbit anti-p73 (pY99) Antibody

• Background:

  Rabbit polyclonal antibody to p73 (pY99)

• Synonyms:

  P73; Tumor protein p73; p53-like transcription factor; p53-related protein

• Host:

  Rabbit

• Reactivity:

  H

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p73. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of p73 (pY99) expression in Jurkat (A), HeLa UV-treated (B) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of p73 (pY99) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  7161

• SwissProt (Human):

  O15350

• CAS Number:

  9007-83-4