Rabbit anti-NGAL Antibody

• Background:

  Rabbit polyclonal antibody to NGAL

• Synonyms:

  HNL; NGAL; Neutrophil gelatinase-associated lipocalin; NGAL; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide of human NGAL

• Clonality:

  Polyclonal

• Applications:

  WB, IHC, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Rabbit

• WB Description:

  Western blot analysis of NGAL expression in SW480 (A), A549 (B) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of NGAL staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  3934

• Gene ID (Mouse):

  16819

• Gene ID (Rat):

  170496

• SwissProt (Human):

  P80188

• SwissProt (Mouse):

  P11672

• SwissProt (Rat):

  P30152

• CAS Number:

  9007-83-4