Rabbit anti-Methyl Lysine Antibody

• Background:

  Rabbit polyclonal antibody to Pan Methyl Lysine

• Host:

  Rabbit

• Immunogen:

  Recombinant protein corresponding to Pan Methyl Lysine.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/1000 - 1/2000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Methyl Lysine expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Methyl Lysine staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• CAS Number:

  9007-83-4