Rabbit anti-Islet-1 Antibody

• Background:

  Rabbit polyclonal antibody to Islet-1

• Synonyms:

  Insulin gene enhancer protein ISL-1; Islet-1

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human Islet-1

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Islet-1 expression in Hela (A), mouse thymus (B), rat thymus (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of Islet-1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  3670

• Gene ID (Mouse):

  16392

• Gene ID (Rat):

  64444

• SwissProt (Human):

  P61371

• SwissProt (Mouse):

  P61372

• SwissProt (Rat):

  P61374

• CAS Number:

  9007-83-4