Rabbit anti-HNF4 alpha/gamma Antibody

• Background:

  Rabbit polyclonal antibody to HNF4 alpha/gamma

• Synonyms:

  HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4; HNF4G; NR2A2; Hepatocyte nuclear factor 4-gamma; HNF-4-gamma; Nuclear receptor subfamily 2 group A member 2

• Host:

  Rabbit

• Reactivity:

  H, M

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HNF4 alpha/gamma. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/50 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of HNF4 alpha/gamma expression in HCT116 (A), CT26 (B), A549 (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of HNF4 alpha/gamma staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  3172; 3174

• Gene ID (Mouse):

  15378; 30942

• SwissProt (Human):

  P41235; Q14541

• SwissProt (Mouse):

  P49698; Q9WUU6

• CAS Number:

  9007-83-4