Rabbit anti-Focal Adhesion Kinase Antibody

• Background:

  Rabbit polyclonal antibody to Focal Adhesion Kinase

• Synonyms:

  FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

• Host:

  Rabbit

• Reactivity:

  H, M, R, C, Mk, Z

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Focal Adhesion Kinase. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Rabbit

• WB Description:

  Western blot analysis of Focal Adhesion Kinase expression in HeLa (A), K562 (B), NIH3T3 (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Focal Adhesion Kinase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  5747

• Gene ID (Mouse):

  14083

• Gene ID (Rat):

  25614

• SwissProt (Human):

  Q05397

• SwissProt (Mouse):

  P34152

• SwissProt (Rat):

  O35346

• CAS Number:

  9007-83-4