Rabbit anti-EPHB1/2 (pY594/604) Antibody

• Background:

  Rabbit polyclonal antibody to EPHB1/2 (pY594/604)

• Synonyms:

  EPHB1; ELK; EPHT2; HEK6; NET; Ephrin type-B receptor 1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2; EPHB2; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; Ephrin type-B receptor 2; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3; EPH-like kinase 5; EK5; hEK5; Renal carcinoma antigen NY-REN-47; Tyrosine-protein kinase TYRO5; Tyrosine-protein kinase receptor EPH-3

• Host:

  Rabbit

• Reactivity:

  H, M, R, C

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPHB1/2 (pY594/604) . The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Rabbit

• WB Description:

  Western blot analysis of EPHB1/2 (pY594/604) expression in A431 (A), HeLa (B), NIH3T3 (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of EPHB1/2 (pY594/604) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

• Gene ID (Human):

  2047; 1969

• Gene ID (Mouse):

  270190

• Gene ID (Rat):

  24338

• SwissProt (Human):

  P54762; P29323

• SwissProt (Mouse):

  Q8CBF3; P54763

• SwissProt (Rat):

  P09759

• CAS Number:

  9007-83-4