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Rabbit anti-eIF3C Antibody

CAT: 0946-YLD2749-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0946-YLD2749-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Rabbit polyclonal antibody to eIF3C
CAS Number
9007-83-4
Synonyms
EIF3S8; Eukaryotic translation initiation factor 3 subunit C; eIF3c; Eukaryotic translation initiation factor 3 subunit 8; eIF3 p110
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human eIF3C
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of eIF3C expression in HepG2 (A), SW620 (B), mouse testis (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of eIF3C staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
8663
Gene ID (Mouse)
56347
Gene ID (Rat)
293484
SwissProt (Human)
Q99613
SwissProt (Mouse)
Q8R1B4
SwissProt (Rat)
B5DFC8