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Rabbit anti-EEF1A1 Antibody

CAT: 0946-YLD2695-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0946-YLD2695-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Rabbit polyclonal antibody to EEF1A1
CAS Number
9007-83-4
Synonyms
EEF1A; EF1A; LENG7; Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human EEF1A1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of EEF1A1 expression in MCF7 (A), Jurkat (B), HepG2 (C), SHSY5Y (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of EEF1A1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1915
Gene ID (Mouse)
13627
Gene ID (Rat)
171361
SwissProt (Human)
P68104
SwissProt (Mouse)
P10126
SwissProt (Rat)
P62630

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