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Rabbit anti-E2F4 Antibody

CAT: 0946-YLD2657-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0946-YLD2657-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Rabbit polyclonal antibody to E2F4
CAS Number
9007-83-4
Synonyms
E2F4; Transcription factor E2F4; E2F-4; E2F5; Transcription factor E2F5; E2F-5
Host
Rabbit
Reactivity
H, M, R, C
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F4. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of E2F4 expression in HeLa (A), K562 (B), Jurkat (C), Raji (D), A431 (E), mouse kidney (F) whole cell lysates.
IHC description
Immunohistochemical analysis of E2F4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1874; 1875
Gene ID (Mouse)
104394; 13559
SwissProt (Human)
Q16254; Q15329
SwissProt (Mouse)
Q8R0K9; Q61502
SwissProt (Rat)
Q62814

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