Rabbit anti-E Cadherin Antibody

• Background:

  Rabbit polyclonal antibody to E Cadherin

• Synonyms:

  CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324

• Host:

  Rabbit

• Reactivity:

  H

• Immunogen:

  Recombinant protein corresponding to human E Cadherin.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/1000 - 1/2000), IH (1/200 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of E Cadherin expression in Hela (A) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of E Cadherin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  999

• SwissProt (Human):

  P12830

• CAS Number:

  9007-83-4