Rabbit anti-CYB5R3 Antibody

• Background:

  Rabbit polyclonal antibody to CYB5R3

• Synonyms:

  DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1

• Host:

  Rabbit

• Reactivity:

  H, D

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CYB5R3. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC, IP

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of CYB5R3 expression in HeLa (A) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of CYB5R3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  1727

• SwissProt (Human):

  P00387

• CAS Number:

  9007-83-4