Rabbit anti-Cortactin (pY421) Antibody

• Background:

  Rabbit polyclonal antibody to Cortactin (pY421)

• Synonyms:

  EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1

• Host:

  Rabbit

• Reactivity:

  H, M

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cortactin (pY421) . The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC, IP

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Cortactin (pY421) expression in HeLa UV-treated (A), NIH3T3 H2O2-treated (B) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Cortactin (pY421) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  2017

• Gene ID (Mouse):

  13043

• SwissProt (Human):

  Q14247

• SwissProt (Mouse):

  Q60598

• CAS Number:

  9007-83-4