Rabbit anti-Complement C3 Antibody

• Background:

  Rabbit polyclonal antibody to Complement C3

• Synonyms:

  CPAMD1; Complement C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human Complement C3

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Complement C3 expression in HepG2 (A), Hela (B), mouse lung (C), rat liver (D) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Complement C3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  718

• Gene ID (Mouse):

  12266

• Gene ID (Rat):

  24232

• SwissProt (Human):

  P01024

• SwissProt (Mouse):

  P01027

• SwissProt (Rat):

  P01026

• CAS Number:

  9007-83-4