Rabbit anti-Asparagine Synthetase Antibody

• Background:

  Rabbit polyclonal antibody to Asparagine Synthetase

• Synonyms:

  TS11; Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human Asparagine Synthetase

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Asparagine Synthetase expression in MCF7 (A), K562 (B), mouse testis (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of Asparagine Synthetase staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  440

• Gene ID (Mouse):

  27053

• Gene ID (Rat):

  25612

• SwissProt (Human):

  P08243

• SwissProt (Mouse):

  Q61024

• SwissProt (Rat):

  P49088

• CAS Number:

  9007-83-4