Human IL-27 Recombinant Rabbit Monoclonal Antibody [PSH03-81] - BSA and Azide free (Capture)

• Description:

  IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of pro-inflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses pro-inflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

• Product Name Alternative:

  Interleukin-27 subunit alpha antibody IL-27 subunit alpha antibody IL-27-A antibody IL27-A antibody Interleukin-30 antibody p28 antibody IL27 antibody IL27A antibody IL30 antibody Interleukin-27 subunit beta antibody IL-27 subunit beta antibody IL-27B antibody Epstein-Barr virus-induced gene 3 protein (EBV-induced gene 3 protein) antibody EBI3 antibody IL27B antibody

• Abbreviation:

  Interleukin-27 subunit alpha IL-27 subunit alpha IL-27-A IL27-A Interleukin-30 p28 IL27 IL27A IL30 Interleukin-27 subunit beta IL-27 subunit beta IL-27B Epstein-Barr virus-induced gene 3 protein (EBV-induced gene 3 protein) EBI3 IL27B

• Swiss Prot:

  Q8NEV9 Human, Q14213 Human

• Cellular Locus:

  Secreted.

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Recombinant protein within Human IL-27 aa 21-229 (Q8NEV9) + Human EBI3 aa 29-243 (Q14213).

• Isotype:

  IgG

• Clone:

  PSH03-81

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  ELISA (Cap)

• Positive Control:

  Recombinant Human IL-27 protein.

• Concentration:

  1 mg/mL

• Dilution:

  ELISA (Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-82] to Human IL-27 (Detector) (HA722049). The reference range value is 61.7-5000 pg/ml.

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  PBS (pH7.4).

• Molecular Weight:

  Predicted band size: 27.5 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3