Anti-Neurofilament (H+L) (Neuronal Marker)(2F11), CF647 conjugate

Anti-Neurofilament (H+L) (Neuronal Marker)(2F11), CF647 conjugate

• Description: This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200. 68kDa neurofilament protein; Light molecular weight neurofilament protein; NEFL; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament protein; light chain; Neurofilament subunit NF-L; Neurofilament triplet L protein; NF-L; NF68
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: NEFH; NEFL
• Gene ID: 4744
• NCBI Gene ID: 198760
• UniProt: P12036
• Cellular Locus: Cytoskeleton
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Human NF-H from isolated brain cells
• Target Antigen: Neurofilament H L
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 2F11
• Conjugation: CF647
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified) | WB (verified)
• Validated Applications: IHC, FFPE, WB
• Field of Research: Cancer, Cytoskeleton, Neuroscience
• Positive Control: IMR-32 cells. Cerebellum or Neuroblastoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 200 kDa & 68 kDa
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years