Anti-Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)(LAG3/3261), CF640R conjugate

• Description:

  LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4 CD8 T cells and NK cell, with shared homology in structure to CD4 molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3 CD4 CD8 T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3 cells) and inhibit induced (i. e. antigen-specific) Treg suppressive activity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD223; FDC protein; LAG-3

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  LAG3

• Gene ID:

  3902

• NCBI Gene ID:

  409523

• UniProt:

  P18627

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human LAG3 protein

• Target Antigen:

  Lymphocyte Activation Gene 3

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  LAG3/3261

• Conjugation:

  CF640R

• Source:

  Animal

• Positive Control:

  Human tonsil or Hodgkin's lymphoma (IHC-P).

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  70 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4