Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker)(CDH1/3256)

Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker)(CDH1/3256)

• Description: Recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2 -dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin ) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CDH1
• Gene ID: 999
• NCBI Gene ID: 461086
• UniProt: P12830
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment of human CDH1 protein (around aa 567-691) (exact sequence is proprietary)
• Target Antigen: CD324 | E-Cadherin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: CDH1/3256
• Conjugation: Purified, BSA-free
• Disease: Tumor
• Source: Animal
• Applications: Flow (verified) | IHC, FFPE (verified)
• Validated Applications: FC, IHC, FFPE
• Positive Control: MCF-7, LS174T, Raji, HT29 or SK-BR3 cells. Prostate or Colon carcinomas.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 120-80 kDa (Mature); 135 kDa (Precursor)
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years