Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker)(CDH1/3256), CF640R conjugate

• Description:

  Recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2 -dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin ) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO)

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CDH1

• Gene ID:

  999

• NCBI Gene ID:

  461086

• UniProt:

  P12830

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human CDH1 protein (around aa 567-691) (exact sequence is proprietary)

• Target Antigen:

  CD324 | E-Cadherin

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  CDH1/3256

• Conjugation:

  CF640R

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow (verified) | IHC, FFPE (verified)

• Validated Applications:

  FC, IHC, FFPE

• Positive Control:

  MCF-7, LS174T, Raji, HT29 or SK-BR3 cells. Prostate or Colon carcinomas.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  120-80 kDa (Mature); 135 kDa (Precursor)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4