Anti-CD95 / FAS / TNFRSF6(FAS/3112)

• Description:

  This MAb specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45 kDa, containing 8 kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  ALPS1A; Apoptosis APO1 antigen 1; Apoptosis-mediating surface antigen FAS; APT1; Delta Fas; Fas AMA; Fas cell surface death receptor; FAS1; FASL receptor; FASLG receptor; FASTM; Surface antigen APO1; Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  FAS

• Gene ID:

  355

• NCBI Gene ID:

  244139

• UniProt:

  P25445

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment (around aa26-96) of human CD95 (FAS) protein (exact sequence is proprietary)

• Target Antigen:

  CD95 | FAS | TNFRSF6

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  FAS/3112

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Positive Control:

  MCF-7 cells (IF/FACS). Human hepatocellular or bladder carcinoma (IHC).

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  38-50 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4