Anti-APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor)(ALi 12-28), CF405S conjugate

• Description:

  Adenomatous Polyposis Coli (APC) is a protein with many cellular functions. It is a tumor suppressor and negative regulator of Wnt signaling. APC has roles in cell adhesion and cell migration. Mutations in APC are assuciated with several diseases. The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Adenomatous Polyposis Coli; APC; CC1; Deleted in polyposis 2.5; Protein Phosphatase 1, Regulatory Subunit 46; DP2; DP3

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  APC

• Gene ID:

  324

• NCBI Gene ID:

  158932

• UniProt:

  P25054

• Cellular Locus:

  Golgi apparatus|Plasma membrane|Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human APC protein (around aa 1-433)

• Target Antigen:

  Adenomatous Polyposis Coli

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  ALi 12-28

• Conjugation:

  CF405S

• Source:

  Animal

• Applications:

  IF (published) | IHC, frozen (published) | IP (published) | WB (published)

• Validated Applications:

  IF, IHC, IP, WB

• Positive Control:

  COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  66 kDa

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1998) 95:3122-3127. (WB, IF, epitope mapping) EMBO Rep (2005) 6:184-190. (IF) BMC Cell Biol (2006) 7: 3. (IF, WB) Int J Mol Med (2008) 21:19-31. (IHC, frozen) J Biol Chem (2012) 287(34): 28552-28563. (IP, WB)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4