Anti-CLEC9A / DNGR1 (Dendritic Cell Marker)(8F9), CF568 conjugate

• Description:

  This MAb recognizes a protein of 27 kDa, identified as CLEC9A (C-type lectin domain family 9 member A). Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  C-type lectin domain family 9 member A (CLEC9A); DNGR1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CLEC9A

• Gene ID:

  283420

• NCBI Gene ID:

  531189

• UniProt:

  Q6UXN8

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  RBL-2H3 cells expressing human DNGR-1 fused to an HA epitope

• Target Antigen:

  CLEC9A | DNGR1

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  8F9

• Conjugation:

  CF568

• Source:

  Animal

• Applications:

  Flow, surface (published) | IF (published)

• Validated Applications:

  FC, IF

• Positive Control:

  MCF-7 cells (FACS/IF).

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  27 kDa

• Additionnal Information:

  ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Clin Invest (2008) 118(6):2098-2110. (IF, Flow) Clinical Translational Immunol (2016) 5:e57. (Flow)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years