Anti-Cyclin B2(X29.2), CF640R conjugate

Anti-Cyclin B2(X29.2), CF640R conjugate

• Description: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: ccnb2; CycB2; Cyclin B2; G2 mitotic specific cyclin B2; HsT17299; MGC108931; MGC140694
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CCNB2
• Gene ID: 9133
• NCBI Gene ID: 194698
• UniProt: O95067
• Cellular Locus: Cytoplasmic
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat, Xenopus
• Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein
• Target Antigen: Cyclin B2
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: X29.2
• Conjugation: CF640R
• Source: Animal
• Applications: IF (published)
• Validated Applications: IF
• Positive Control: HeLa cells. Human cervical tissue (IHC).
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 51 kDa
• Additionnal Information: ELISA: For coating, order antibody without BSA; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. PLoS ONE 3(4): e1871. (IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years