Anti-TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer)(TIGIT/3017), Biotin conjugate

Anti-TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer)(TIGIT/3017), Biotin conjugate

• Description: TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4 T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9 (VSIG9); V-set and transmembrane domain containing 3 (VSTM3); Washington University cell adhesion molecule (WUCAM)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TIGIT
• Gene ID: 201633
• NCBI Gene ID: 421750
• UniProt: Q4951
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)
• Target Antigen: TIGIT | VSIG9 | VSTM3
• Clonality: Monoclonal
• Isotype: IgG2c κ
• Clone: TIGIT/3017
• Conjugation: Biotin
• Source: Animal
• Applications: Flow (verified) | IHC, FFPE (verified)
• Validated Applications: FC, IHC, FFPE
• Positive Control: MOLT-4, Prostate or Colon Carcinoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 26 kDa
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years