Anti-VISTA / B7-H5 / VSIR (Negative Regulator of Immune Response)(VISTA/3007), CF647 conjugate

• Description:

  VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  B7H5; DD1alpha; GI24; PD-1H; PDCD1 homolog; Platelet receptor Gi24; PP2135; Stress-induced secreted protein 1 (SISP1); V domain Ig suppressor of T cell activation (VISTA); V Set Immuno-Regulatory Receptor

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  VSIR

• Gene ID:

  64115

• NCBI Gene ID:

  47382

• UniProt:

  Q9H7M9

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human VISTA protein

• Target Antigen:

  B7-H5 | VISTA | VSIR

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  VISTA/3007

• Conjugation:

  CF647

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Positive Control:

  Spleen or Placenta.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  34 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4