Anti-Cyclin B1 (G2- & M-phase Cyclin)(CCNB1/2978R), CF640R conjugate

Anti-Cyclin B1 (G2- & M-phase Cyclin)(CCNB1/2978R), CF640R conjugate

• Description: Recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CCNB; CCNB1; CCNB1_HUMAN; G2 Mitotic Specific Cyclin B1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CCNB1
• Gene ID: 891
• NCBI Gene ID: 23960
• UniProt: P14635
• Cellular Locus: Cytoplasmic
• Host: Rabbit
• Species Reactivity: Hamster, Human, Mouse
• Immunogen: Recombinant full-length human CCNB1 protein
• Target Antigen: Cyclin B1
• Clonality: Recombinant Monoclonal
• Isotype: IgG
• Clone: CCNB1/2978R
• Conjugation: CF640R
• Source: Animal
• Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 55-62 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• References & Citations: Minemoto Y et al. Exp Cell Res 262:37-48 (2001) |Garner AP et al. Oncogene 21:8089-104 (2002)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years