Anti-RNA Polymerase II(CTD4H8), Biotin conjugate

Anti-RNA Polymerase II(CTD4H8), Biotin conjugate

• Description: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1, 840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit; DNA-directed RNA polymerase II subunit RPB1; hRPB220; hsRPB1; POLR2; Polr2a; POLRA; Polymerase (RNA) II (DNA directed) polypeptide A 220kDa; RNA polymerase II subunit B1; RPBh1; RpIILS; RPO2; RPOL2
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: POLR2A
• Gene ID: 5430
• NCBI Gene ID: 270017
• UniProt: P24928
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
• Target Antigen: RNA Polymerase II
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: CTD4H8
• Conjugation: Biotin
• Source: Animal
• Applications: IHC, FFPE (verified) | WB (verified)
• Validated Applications: IHC, FFPE, WB
• Positive Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 192-253 kDa
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years