Anti-CD29 (Stem Cell Marker)(12G10), CF405S conjugate

Anti-CD29 (Stem Cell Marker)(12G10), CF405S conjugate

• Description: Recognizes CD29. Binds to the cell membrane of T- and B- lymphocytes, monocytes, platelets, fibroblasts, endothelial cells, mast cells and smooth muscle cells. CD29 is an adhesion receptor and co-stimulatory molecule. This antigen was initially characterized as GPIIa on platelets and as the common b1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin alpha subunits. It also mediates adhesion to invasion and thrombospondin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Fibrinogen Receptor beta subunit; FNRB; Glycoprotein IIa (GPIIA); Integrin beta-1 (ITGB1); integrin VLA-4 beta subunit; MDF2; MSK12; Very Late Activation Protein beta Polypeptide; VLA-4 subunit beta; VLA-BETA; VLAB
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ITGB1
• Gene ID: 3688
• NCBI Gene ID: 643813
• UniProt: P05556
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Purified human beta 1 integrin from HT1080 fibrosarcoma cell extract.
• Target Antigen: CD29
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 12G10
• Conjugation: CF405S
• Source: Animal
• Applications: Flow, surface (published) | Functional studies (published) | IF (published) | IP (published)
• Validated Applications: FC, IF, IP
• Positive Control: HT1080 whole cell lysate. HeLa cell line.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 110 kDa (non-reduced) & 130 kDa (reduced)
• Additionnal Information: Does not react with Mouse or Rat; ELISA: For coating use Ab at 1-5 ug/mL, order Ab without BSA; Optimal dilution for a specific application should be determined by user.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. FEBS Lett (1995) 363: 118-122. (functional studies) J Cell Sci (2005) 118: 4009-4016. (IP) Traffic (2012) 13: 610-625. (Flow, IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years