Anti-WIPI2(2A2), CF405S conjugate

Anti-WIPI2(2A2), CF405S conjugate

• Description: WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al. , 2004 ). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WIPI2; WIPI49 like protein 2
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: WIPI2
• Gene ID: 26100
• NCBI Gene ID: 122363
• UniProt: Q9Y4P8
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant fragment (around aa 418-436) of human WIPI2 protein (exact sequence is proprietary)
• Target Antigen: WIPI2
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 2A2
• Conjugation: CF405S
• Source: Animal
• Applications: IF (published) | IP (published) | WB (published)
• Validated Applications: IF, IP, WB
• Positive Control: HEK293 cells. Human or mouse placenta, skeletal muscle or testis.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 49 kDa
• Additionnal Information: ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Autophagy (2010) 6(4):506-522. (IF) Brain (2019) 142(5): 1242-1254. (IF, IP, WB)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years