Anti-CD59 / Complement Regulatory Protein / Protectin (MACIF/2867R)

Anti-CD59 / Complement Regulatory Protein / Protectin (MACIF/2867R)

• Description: This antibody reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI; Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: 20kDa homologous restriction factor (HRF20); Complement regulatory protein; Human leukocyte antigen MIC11; MAC-inhibitory protein (MACIP); Membrane attack complex inhibition factor (MACIF); Membrane inhibitor of reactive lysis; MIRL; MSK21; Protectin; T cell activating protein
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD59
• Gene ID: 966
• NCBI Gene ID: 278573; 709466; 710641
• UniProt: P13987
• Cellular Locus: Plasma membrane
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Recombinant full-length human CD59 protein
• Target Antigen: CD59
• Clonality: Recombinant Monoclonal
• Isotype: IgG
• Clone: MACIF/2867R
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Hematology, Immunology
• Positive Control: Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 20 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Does not react with baboon or horse|Flow cytometry: 0.5-1 ug/million cells|Functional Studies Order Ab without Azide|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years