Anti-Fibronectin(Fn-3)

• Description:

  Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  FN1

• Gene ID:

  2335

• NCBI Gene ID:

  203717

• UniProt:

  P02751

• Cellular Locus:

  Extracellular matrix

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  FR5 cells, derived by SV40 transformation of human mammary epithelial cells.

• Target Antigen:

  Fibronectin

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  Fn-3

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IF (published) | WB (published)

• Validated Applications:

  IF, WB

• Field of Research:

  Cell adhesion, Extracellular matrix

• Positive Control:

  SW156 cells. Kidney.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  220 kDa (monomer); 440 kDa (dimer)

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Am J Physiol Cell Physiol (2002) 282: C654-C664. (IF; WB) Meth Mol Biol (2009) https://doi.org/10.1007/978-1-59745-413-1_18 (IF) J Tissue Eng Regen Med. (2020) 14:761-773. (IF, frozen tissue sections)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4