Mouse IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH03-31] - BSA and Azide free (Capture)

• Description:

  The Interleukin 1 (IL-1) family of proteins consists of IL-1 alpha, IL-1 beta, and the IL-1 receptor antagonist (IL-1ra). IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions. IL-1 is not produced by unstimulated cells of healthy individuals with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. However, in response to inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulindependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid (aa) level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into mature proteins of approximately 17.5 kDa. Cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. Neither IL-1 alpha nor IL-1 beta contains a typical hydrophobic signal peptide, but evidence suggests that these factors can be secreted by non-classical pathways. A portion of unprocessed IL-1 alpha can be presented on the cell membrane and may retain biological activity. The precursor form of IL-1 beta, unlike the IL-1 alpha precursor, shows little or no biological activity in comparison to the processed form. Both unprocessed and mature forms of IL-1 beta are exported from the cell.

• Product Name Alternative:

  Catabolin antibody H1 antibody IFN beta inducing factor antibody IL 1 antibody IL 1 beta antibody IL-1 beta antibody IL1 antibody IL1 BETA antibody IL1B antibody IL1B_HUMAN antibody IL1F2 antibody Interleukin 1 beta antibody Interleukin 1 beta precursor antibody interleukin 1, beta antibody Interleukin-1 beta antibody OAF antibody Osteoclast activating factor antibody OTTHUMP00000162031 antibody Preinterleukin 1 beta antibody Preinterleukin beta antibody Pro interleukin 1 beta antibody

• Abbreviation:

  Interleukin-1 beta IL-1 beta Il1b

• Swiss Prot:

  P10749 Mouse

• Cellular Locus:

  Secreted

• Host:

  Rabbit

• Species Reactivity:

  Mouse

• Immunogen:

  Recombinant protein within Mouse IL-1 beta aa 118-269 (P10749).

• Isotype:

  IgG

• Clone:

  PSH03-31

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  ELISA (Cap)

• Positive Control:

  Recombinant Mouse IL-1 beta protein.

• Concentration:

  1 mg/mL

• Dilution:

  ELISA (Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-32] to Mouse IL1 beta (Dctector) (HA721977) and recombinant standard Mouse IL1 beta protein (HA210706) as the standard. The reference range value is 37-3000pg/ml.

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  PBS (pH7.4).

• Molecular Weight:

  Predicted band size: 30.9 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3