Anti-SMAD4 (Pancreatic Adenocarcinoma Marker)(SMAD4/2524)

• Description:

  Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4. Upon ligand binding, the receptors of the TGF- family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. SMAD4 is absent in approximately 80% of pancreatic adenocarcinoma, but rarely in endometrial, colorectal, ovarian, lung, breast adenocarcinomas, and malignant melanom. SMAD4 is an important marker for confirming a diagnosis of pancreatic adenocarcinoma. Patients with pancreatic adenocarcinomas with SMAD4 protein expression had significantly longer survival than SMAD4 negative patients. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Deleted in Pancreatic Carcinoma 4 (DPC4); JIP; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4 (MADH4); Mothers against DPP homolog 4; MYHRS; SMAD4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  STAT6

• Gene ID:

  4089

• NCBI Gene ID:

  75862

• UniProt:

  Q13485

• Cellular Locus:

  Nucleus & cytoplasm

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human STAT6 protein

• Target Antigen:

  SMAD4

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  SMAD4/2524

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Source:

  Animal

• Positive Control:

  Jurkat or A431 cells. Pancreatic Carcinoma.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  61 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4