Anti-CD3e (T-Cell Marker)(UCHT1), CF640R conjugate

Anti-CD3e (T-Cell Marker)(UCHT1), CF640R conjugate

• Description: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD3E
• Gene ID: 916
• NCBI Gene ID: 3003
• UniProt: P07766
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Chimpanzee, Human
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
• Target Antigen: CD3e
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: UCHT1
• Conjugation: CF640R
• Disease: Tumor
• Source: Animal
• Applications: Functional studies (published) | ELISA (published) | Flow, surface (verified) | IF (verified) | IP (published) | WB (published)
• Validated Applications: ELISA, FC, IF, IP, WB
• Positive Control: Human peripheral blood. Jurkat cells. Tonsil or lymph node.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 20 kDa
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. _x000D_ 1. Blood (1988) 71(3): 603-612. (immunofluorescence, western)_x000D_ 2. J Immunol Meth (1988) 106(1): 127-133. (ELISA)_x000D_ 3. J Exp Med (1991) 173: 7-17. (immunoprecipitation)_x000D_ 4. J Immunol (2002) 168: 3235-3241. (functional studies)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years