Anti-Bcl-X (Apoptosis Marker)(BCL2L1/2406), CF488A conjugate

• Description:

  Bcl-x belongs to the Bcl-2 family of proteins and plays a crucial role in apoptosis. Bcl-X has two isoforms: Bcl-XL (long), a 26 kDa protein which suppresses cell death, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2 and promotes apoptosis. This MAb reacts with both Bcl-XS and Bcl-XL proteins, and shows no cross-reaction with Bcl-2 or Bax proteins.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Bcl-x; Bcl-2-like protein 1; BCL2L1; Apoptosis regulator Bcl-X

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  BCL2L1

• Gene ID:

  598

• NCBI Gene ID:

  516966

• UniProt:

  Q07817

• Cellular Locus:

  Cytoplasmic|Endoplasmic reticulum|Mitochondria

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human BCL2L1 protein fragment (around aa 1-135) (exact sequence is proprietary)

• Target Antigen:

  Bcl-x

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  BCL2L1/2406

• Conjugation:

  CF488A

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Apoptosis, Cancer

• Positive Control:

  Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  27 kDa

• Additionnal Information:

  ELISA: For coating use Ab at 1-5 ug/mL, order Ab without BSA; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years