Anti-NME1 / nm23-H1 / NDPK-A (Suppressor of Metastasis)(CPTC-NME1-2), CF405S conjugate

Anti-NME1 / nm23-H1 / NDPK-A (Suppressor of Metastasis)(CPTC-NME1-2), CF405S conjugate

• Description: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP-K). In humans, NDP kinase A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human C-Myc promotor. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: AWD; GAAD; Granzyme A activated DNase; Metastasis inhibition factor NM23; NB; NBS; NDP kinase A; NM23 long variant; included; NM23H1B; NME/NM23 nucleoside diphosphate kinase 1; NME1-NME2 spliced read-through transcript; included; Non-metastatic protein 23; homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: NME1
• Gene ID: 4830
• NCBI Gene ID: 463456
• UniProt: P15531
• Cellular Locus: Nucleus & cytoplasm
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant full-length human NME1 protein
• Target Antigen: NDPK-A | nm23-H1 | NME1
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: CPTC-NME1-2
• Conjugation: CF405S
• Source: Animal
• Applications: WB (verified)
• Validated Applications: WB
• Positive Control: HepG2 and HeLa whole cell lysates; heart, brain, placenta and lung carcinoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 23 kDa
• Additionnal Information: Western blot 0.5-1.0 ug/mL; Optimal dilution for a specific application should be determined.
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years