Anti-HLA-DR (MHC II)(TAL 1B5), CF647 conjugate

• Description:

  Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4 helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC class II/ dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the sub- sequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain ( -Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  DR alpha chain; DRB1; DRB4; HLA class II histocompatibility antigen DR alpha chain; HLA DR1B; HLA DR3B; HLA DRA; HLA-DRA; HLADR4B; HLADRA1; HLADRB; Major histocompatibility complex class II DR alpha; Major histocompatibility complex class II DR beta 1/3/4/5

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  HLA-DR

• Gene ID:

  3122

• NCBI Gene ID:

  520048

• UniProt:

  P01903

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Target Antigen:

  HLA-DR

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  TAL 1B5

• Conjugation:

  CF647

• Source:

  Animal

• Applications:

  Flow (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IHC, FFPE, WB

• Positive Control:

  Raji, Ramos or Daudi whole cell lysates. Spleen, tonsil or liver tissue lysates. Human melanoma, tonsil or lymph node.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  36 kDa (alpha chain); 27 kDa (beta chain); 63 kDa (HLA-DR dimer)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4