Anti-CD8b, Mouse(H35-17.2), Biotin conjugate

• Description:

  This rat monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD8b antigen; CD8 antigen beta polypeptide 1 (p37); CD8 beta; CD8B1; Leu2; Ly3; LYT3; P37; T lymphocyte surface glycoprotein beta chain; T-cell surface glycoprotein CD8 beta chain

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  Cd8b1 (Mouse)

• Gene ID:

  12526

• NCBI Gene ID:

  3331

• UniProt:

  P10300

• Cellular Locus:

  Plasma membrane

• Host:

  Rat

• Species Reactivity:

  Mouse

• Immunogen:

  Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c

• Target Antigen:

  CD8b,

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  H35-17.2

• Conjugation:

  Biotin

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published)

• Validated Applications:

  FC, Functional assays

• Positive Control:

  MPBMC's or Thymus.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  37 kDa

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Infection Immunity (1994) 62(5): 1820-1829. (functional studies) Meth Mol Biol vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_2 (Flow, surface)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4