Anti-PD1, Mouse(RMP1-14), CF568 conjugate

• Description:

  PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B-lymphocytes and myeloid cells. Additionally, Pdcd1mRNA is expressed in developing B-lymphocytes during the pro-B-cell stage. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production co-stimulated by macrophages (but not by dendritic cells and B cells)._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  CD279; CD279 antigen; PD1; PDCD1; Programmed cell death 1; Programmed cell death protein 1; Systemic lupus erythematosus susceptibility 2 (SLEB2)

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene ID:

  18566

• NCBI Gene ID:

  5024

• UniProt:

  Q02242

• Cellular Locus:

  Plasma membrane

• Host:

  Rat

• Species Reactivity:

  Mouse

• Immunogen:

  PDCD1 transfected BHK cells

• Target Antigen:

  PD1

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  RMP1-14

• Conjugation:

  CF568

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published)

• Validated Applications:

  FC, Functional assays

• Positive Control:

  Spleen, Thymus or Lymph Node.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  ~50-55 kDa

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (2005) 175:1586-1592. (Flow; functional studies) Cancer Res (2014) 74(14): 3652-8. (functional studies) Cancer Discov (2017) 7(7): 694-703. (functional studies)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4