Anti-IL-4 / Interleukin 4(IL4/1597), CF488A conjugate

• Description:

  Interleukin-4 (IL-4), also designated B cell stimulatory factor-1, is a glycosylated cytokine secreted by activated T lymphocytes, basophils and mast cells. The secreted IL-4 protein promotes the growth and differentiation of cells that participate in immune defense by favoring such events as the expansion of the Th2 lineage relative to Th1 cells. These T helper cell subsets are defined by their pattern of cytokine secretion: Th1 cells secrete IL-2, TNF, while Th2 cells secrete IL-4, IL-5 and IL-10. Another key immunological function of IL-4 is to induce immunoglobulin class switching. IL-4 has been shown to induce the production of IgE and enhance IgG4 secretion by B cells, but suppress the production of IgM, IgA, IgG1, IgG2 and IgG3. It has been determined that Stat6 is rapidly tyrosine phosphorylated following stimulation of IL-3 or IL-4, but is not detectably phosphorylated following stimulation with IL-2, IL-12 or erythropoietin._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; BCGF1; Binetrakin; BSF1; IGG1 induction factor; Il4e12; Interleukin 4 variant 2; Interleukin 4; isoform 1Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  HIL41

• Gene ID:

  3565

• NCBI Gene ID:

  73917

• UniProt:

  P05112

• Cellular Locus:

  Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human IL-4 protein

• Target Antigen:

  IL-4 , | Interleukin 4

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  IL4/1597

• Conjugation:

  CF488A

• Source:

  Animal

• Positive Control:

  Spleen.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  18 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4