Anti-HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)(ESEE122)

Anti-HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)(ESEE122)

• Description: HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc. ) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS superfamily 1 (MOP1); PAS domain-containing protein 8 (PASD8)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: HIF1A
• Gene ID: 3091
• NCBI Gene ID: 597216
• UniProt: Q16665
• Cellular Locus: Nucleus & cytoplasm
• Host: Mouse
• Species Reactivity: Cow, Dog, Human, Mouse, Rat
• Immunogen: GST-human HIF-1A amino acids 329-530 fusion protein
• Target Antigen: HIF1 Alpha
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: ESEE122
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (published) | WB (published)
• Validated Applications: IHC, FFPE, WB
• Positive Control: Cobalt chloride treated HeLa cells. Colon, Renal, Breast or Bladder Carcinomas.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 92-110 kDa
• Additionnal Information: ELISA: For coating, purchase antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer (2006) 107(4): 757-766. (IHC, FFPE) Biol Pharm Bull (2017) 40(1): 82-87. (WB)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years